bdSample preparation guides
How to collect, preserve and send your samples so they pass quality control first time. Read online or download the PDF. Some guides are in Bangla.
Bacteria & isolates
Preparing bacterial cultures for DNA extraction and PCR
How to grow, check and harvest a pure bacterial culture so the DNA is clean and the PCR or sequencing works first time.
Lab protocol: bacterial DNA extraction, 16S PCR and gel check
A simple phenol–chloroform DNA extraction, 16S rRNA PCR (27F/1492R) and agarose gel protocol for labs that prepare their own DNA or PCR products before sending them to us.
Bacterial DNA for whole genome sequencing
How to grow, harvest and extract high-molecular-weight bacterial DNA for Illumina or long-read whole genome sequencing, with quality targets and troubleshooting.
Pure cultures for MALDI-TOF and VITEK 2 identification
How to prepare and send fresh, pure microbial isolates for identification by MALDI-TOF MS or VITEK 2, with or without antimicrobial susceptibility testing (AST).
Bacteriophage DNA for whole genome sequencing
How to prepare purified, host-free phage DNA for whole genome sequencing, the DNA quality we need, and the host information to send with it.
Genomes & tissues
Fungal cultures and DNA for whole genome sequencing
How to keep fungal cultures pure and extract clean, high-molecular-weight DNA for fungal whole genome sequencing, with the DNA quality targets.
RNA virus whole genome sequencing: animal and plant samples (Bangla)
প্রাণী বা উদ্ভিদের RNA virus-এর whole-genome sequencing-এর জন্য আক্রান্ত tissue RNAlater-এ সংরক্ষণ অথবা cDNA প্রস্তুত করে BD Genomes-এ পাঠানোর নির্দেশিকা।
Collecting samples for genome and exome sequencing
How to collect and preserve blood, tissue, swabs and cell samples so the DNA is intact and pure enough for whole genome, resequencing, exome or mitogenome sequencing.
Microbiome & eDNA
Collecting eDNA from water, soil, sediment and air
How to collect, filter and preserve environmental DNA samples for 16S amplicon, metabarcoding and shotgun metagenomics, with contamination controls.
Collecting fish eDNA from water for metabarcoding (Bangla)
নদী, পুকুর, হাওর-বিল থেকে পানি সংগ্রহ, filtration বা centrifugation-এর মাধ্যমে eDNA ঘনীভূতকরণ এবং Fish Metabarcoding-এর জন্য filter বা pellet সংরক্ষণের নির্দেশিকা।
Collecting gut content (e.g. caecum) for microbiome sequencing
How to collect, preserve and ship animal gut content for 16S amplicon or shotgun metagenomics without contamination or DNA degradation.
RNA & gene expression
Bacterial culture samples for RNA-Seq (Bangla)
নির্দিষ্ট condition-এ bacterial gene expression (DEG, stress, virulence, antibiotic response) বিশ্লেষণের জন্য culture থেকে cell pellet সংগ্রহ, RNAlater-এ সংরক্ষণ ও BD Genomes-এ পাঠানোর নির্দেশিকা।
Tree and plant root samples for RNA-Seq
How to dig, clean and preserve root tissue (liquid nitrogen or RNAlater) so the RNA stays intact for RNA-Seq, with quality targets for difficult, polyphenol-rich roots.
Tissue samples in RNAlater for qPCR gene expression
A short SOP for collecting animal tissue into RNAlater for RT-qPCR or RNA-Seq: piece size, RNAlater volume, incubation and storage.
Preparing RNA and cDNA for RNA-Seq
How to collect, preserve and extract intact, DNA-free RNA from cells and tissues, make cDNA, and meet the quality targets for RNA-Seq.
Primers & PCR
Primer design for PCR and Sanger sequencing
How to design primers that give a single sharp band and clean Sanger reads: target choice, design tools, key parameters, dimer checks and troubleshooting.
Primer reconstitution, dilution and storage (Bangla)
GeneCreate / Sangon Biotech থেকে আসা lyophilized প্রাইমার গ্রহণ, 100 µM stock ও 10 µM working solution তৈরি, সংরক্ষণ এবং PCR-এ ব্যবহারের ধাপে ধাপে নির্দেশিকা।
Have a sequencing project in mind?
Tell us your organism, sample type and what you want to find out. We will reply with the right service and a quote.
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