Transcriptomics

RNA sequencing

Measure which genes are switched on, and by how much, under different conditions. RNA sequencing is used to study stress responses, infection, development and disease.

RNA sequencing
Turnaround
4–5 weeks after QC
Price
From BDT 35,000 per sample
Platforms
Illumina NovaSeq 6000

What we offer

  • Transcriptomics (RNA-Seq)
  • mRNA-Seq
  • Gene expression profiling
  • mRNA analysis
  • Small RNA analysis

Who it is for

Researchers comparing gene expression between treatments, tissues, strains or time points.

What you receive

  • Raw data (FASTQ)
  • Quality-control report
  • Standard RNA-Seq analysis (included for 30+ samples)

Services & prices

Choose your service, download its sample sheet, fill it in and attach it to the quote form. Prices are per sample unless stated and exclude VAT and tax. The final price is confirmed in your quotation, with no hidden costs and no advance payment.

Transcriptome sequencing (RNA-Seq)

BDT 35,000 per sample

From cDNA. From raw samples in RNAlater: BDT 40,000 per sample. If a sample fails QC, RNA extraction and cDNA synthesis cost BDT 5,000 extra. Bulk price for more than 20 samples.

Turnaround: 4–5 weeks after sample QC

  • cDNA made with oligo(dT) primer: 20 ng/µL, more than 30 µL per sample
  • Or tissue preserved in RNAlater
  • Store at −80 °C; ship on ice
  • Illumina NovaSeq 6000, PE150, more than 6 Gb per sample

Analysis: Standard RNA-Seq analysis included for 30 or more samples submitted together; available for smaller projects at extra cost.

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Gene expression by RT-qPCR

Priced per project

Charged per item: RNA extraction, cDNA synthesis, primers and qPCR reactions (samples × genes). Example: 24 raw samples × 5 genes, from RNA extraction to report, BDT 313,800 (about BDT 13,075 per sample). If a sample fails RNA QC, repeat extraction and cDNA synthesis cost BDT 2,200. Discounts for more than 50 samples.

Turnaround: 2–3 weeks after sample QC

  • Send cDNA, or fresh tissue, cell pellets or samples in RNAlater
  • Ship on ice or cold packs; store at −80 °C
  • Includes a housekeeping (reference) gene; we can design and synthesise the primers

Analysis: Ct values, ΔCt and ΔΔCt, fold change, statistical tests and publication-ready graphs.

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Not sure which option fits? Message us on WhatsApp. Packing and shipping steps are on How to order.

Frequently asked questions

How should I preserve RNA samples?

Send cDNA made with oligo(dT) primer, stored at −80 °C and shipped on ice, or tissue preserved in RNAlater. If a sample fails quality control, RNA extraction and cDNA synthesis cost BDT 5,000 extra per sample.

How many replicates do I need?

At least three biological replicates per condition are recommended for reliable results.

Can you do the bioinformatics?

Yes. Standard RNA-Seq analysis is included for projects of 30 or more samples submitted together. For smaller projects it is available at extra cost.

Ready to start?

Have a sequencing project in mind?

Tell us your organism, sample type and what you want to find out. We will reply with the right service and a quote.

Or call +880 1308-222824